| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Brucella ceti | ACCTTGTCGGTGCTTGCA | AAGCACCGGATTGCCATGA | 59.81 | 60.00 | 240 | 82 |
100.00%
|
50.00%
|
| Primer 12 | Brucella ceti | ACCTTGTCGGTGCTTGCA | CAATGTGGTGCCGTTGACG | 59.81 | 60.08 | 213 | 82 |
100.00%
|
50.00%
|
| Primer 13 | Brucella ceti | ACAGCGATGGATCATGCCC | AAAACCGCAAGCTGCACG | 60.23 | 59.97 | 209 | 82 |
100.00%
|
50.00%
|
| Primer 14 | Brucella ceti | TTCCATTGCCGAACGTGC | TTTGCCAGCATTGCGAGC | 59.05 | 59.74 | 198 | 80 |
100.00%
|
50.00%
|
| Primer 15 | Brucella ceti | TCGAAGCAGCCCACATGA | AACTTGTTGCCGTTCGCG | 58.93 | 59.67 | 186 | 80 |
100.00%
|
50.00%
|
| Primer 16 | Brucella ceti | TCGAAGCAGCCCACATGAG | AACTTGTTGCCGTTCGCG | 60.08 | 59.67 | 186 | 80 |
100.00%
|
50.00%
|
| Primer 17 | Brucella ceti | CAGATCGAAGCAGCCCACA | AACTTGTTGCCGTTCGCG | 60.08 | 59.67 | 190 | 80 |
100.00%
|
50.00%
|
| Primer 18 | Brucella ceti | TCAGATCGAAGCAGCCCAC | AACTTGTTGCCGTTCGCG | 59.78 | 59.67 | 191 | 80 |
100.00%
|
50.00%
|
| Primer 19 | Brucella ceti | GTCAGATCGAAGCAGCCCA | AACTTGTTGCCGTTCGCG | 59.78 | 59.67 | 192 | 80 |
100.00%
|
50.00%
|
| Primer 20 | Brucella ceti | AGCGTATCAGCCATGCAGG | AACTTGTTGCCGTTCGCG | 60.23 | 59.67 | 169 | 80 |
100.00%
|
50.00%
|